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This IgG should really neutralize the more routinely encountered HAMA.[ninety] Methods the laboratory can take to evaluate this risk involve utilizing a bigger dilution in the sample having a reagent containing the non-immune mouse IgG or carrying out the analysis over a in another way configured assay, preferably one particular employing distinct species of reagent antibodies.[ninety one]
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The binding of heparin to cTnI might reduce immunoreactivity, depending on the heparin concentration in sample collection tubes (e.g., heparin in a concentration of ninety U/mL is described to result in a decrease of close to twenty% in cTnI concentration).[33] The influence of heparin on cTnI immunoassays may very well be induced by variations in the sample matrix.
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